Simultaneous Estimation of Paracetamol, Chlorzoxazone and Aceclofenac in Pharmaceutical Formulation by Hplc Method

A rapid and sensitive high performance liquid chromatography with UV detection method was developed and validated for the quantification of paracetamol, chlorzoxazone and aceclofenac in dosage forms. The chromatographic separation was carried out on a Phenomenex Luna C18 column using a mixture of acetonitrile-0.05M disodium hydrogen orthophosphate (65:35) (pH adjusted to 3.0 using 10% orthophosphoric acid) as mobile phase with UV detection at 271 nm. The calibration curve was linear in the concentration range of 20-100 μg/ml for paracetamol, 20-100 μg/ml for chlorzoxazone and 4-20 μg/ml for aceclofenac. The lower limit of detection was found to be 0.9 μg, 1.81 μg, 0.9 μg for paracetamol, chlorzoxazone, aceclofenac respectively. The method was successfully used for quantitative determination of Hifenac-MR tablets.